中国科学院武汉病毒研究所，研究员、硕士生导师 / Professor, Wuhan Institute of Virology, Chinese Academy of Sciences.

学习经历 / Education

1998-2001：中科院武汉病毒研究所和沈阳应用生态研究所 微生物学 博士 / Ph.D. in Microbiology, Wuhan Institute of Virology and Shenyang Institute of Applied Ecology, Chinese Academy of Sciences

1995-1998：沈阳农业大学 食品科学 硕士 / M.S. in Food Science, Shenyang Agriculture University, China.

1991-1995：沈阳农业大学 食品科学 学士 / B.S. in Food Science, Shenyang Agriculture University, China.


工作经历 / Work Experience

2007-至今：中国科学院武汉病毒研究所，研究员/ Professor, Wuhan Institute of Virology, Chinese Academy of Sciences.

2002-2007：中国科学院武汉病毒研究所，副研究员/ Associate professor, Wuhan Institute of Virology, Chinese Academy of Sciences.

2001-2002：中国科学院武汉病毒研究所，助理研究员/ Assistant professor, Wuhan Institute of Virology, Chinese Academy of Sciences.


学术兼职 / Committee and Broad Memberships

1. 湖北省遗传学会理事 / Broad Member, Hubei Society for Genetics

研究方向 / Research Interests

1． 生物分子识别：开展核酸、蛋白质等生物分子识别及生物传感研究，发掘新的病原微生物分析与检测的分子识别原件，并通过基因操纵对分子识别元件进行定向改造，以发展新的病原微生物分析方法和检测技术 / Molecular Recognition: conducting the fundamental researches in molecular recognition and biosensing, exploring new molecular recognition elements for the analysis and detection of pathogenic microbes by genetically engineering the biological elements such as enzymes, functional proteins and genes.

2． 分子酶学：开展病原微生物致病和治疗相关的重要蛋白质和酶的结构与功能研究，为阐释致病机理、耐药机理及发现新的药物靶标提供重要的参考依据；采用理性设计和体外分子进化等手段，对重要工业用酶和分析用酶进行定向改造和加工，发展新的或性能更优良的酶/ Molecular Enzymology: exploring structure and function of proteins and enzymes that involve in disease causing and drug resistance of Bacillus tuberculosis and other severe pathogens , genetically engineering enzymes by rational design and directed evolution in vitro, for the purpose of developing new catalysts with better performance.

近期代表性学术论文 / Selected Recent Publications

1. Yang W, Zhou YF, Dai HP, Bi LJ, Zhang ZP, Zhang XH, Leng Y, Zhang XE*(2008). Application of methyl parathion hydrolase (MPH) as a labeling enzyme. Anal. Bioanal. Chem. 390: 2133-2140. (Co-first author)

2. Zhang H, Deng JY, Bi LJ, Zhou YF, Zhang ZP, Zhang CG, Zhang Y, Zhang XE*(2008). Characterization of Mycobacterium tuberculosis nicotinamidase/pyrazinamidase. FEBS J. 275: 753-762.

3. Hang X, Zhang XE*, Zhou YF, Zhang ZP, Anothony E.G.Cass (2007). Directed evolution of the 5’-untranslated region of the phoA gene in Escherichia coli simultaneously yields a stronger promoter and a stronger Shine-Dalgarno sequence Biotechnol.j. 1:1275-1282.

4. You F, Zhou YF, Zhang XE*, Huang Z, Bi LJ, Zhang ZP, Wen JK (2006). A New Cell-free Bioassay for Rapid and Sensitive Measurement of Dioxins Based on Fluorescence Enhancement of FITC Labeled DNA Probe, Anal.l Chem, 78(20):7138-7144.

5. Guo YC, Zhou YF, Zhang XE*, Zhang ZP, Qiao YM, Wen JK (2006). Phage display mediated immuno-PCR, Nucleic Acids Research, 34(8): e62.

6. Huang YY ,Deng JY, Gu J, Zhang ZP, Maxwell A, Bi LJ, Chen YY, Zhou YF, Yu ZN, Zhang XE*(2006). The key DNA-binding residues in the C-terminal domain of Mycobacterium tuberculosis DNA gyrase A subunit (GyrA) Nucleic Acids Research 34(19): 5650-5659.

7. Dong YJ, Bartlam M, Sun L, Zhou YF, Zhang ZP, Zhang CG, Rao ZH* and Zhang XE* (2005). Crystal Structure of Methyl Parathion Hydrolase from Pseudomonas sp. WBC-3, Journal of Molecular Biology, 353 (3): 655-663.

8. Bi LJ, Zhou YF, Zhang XE*, Deng JY, Wen JK, Zhang ZP (2005), Construction and characterization of different Muts fusion proteins as recognition elements of DNA chip for detection of DNA mutations. Biosensors & Bioelectronics, 21(1): 135-144.

9. Cui ZQ, Zhang ZP, Zhang XE*, Wen JK, Zhou YF, Xie WH (2005). Visualizing the dynamic behavior of poliovirus plus-strand RNA in living host cells. Nucleic Acids Research, 33(10): 3245-3252.

10. Wang SH, Wen JK, Zhou YF, Zhang ZP, Yang RF, Zhang JB, Chen J and Zhang XE* (2004), Identification and characterization of Bacillus anthracis by multiplex PCR on DNA chip, Biosensors & Bioelectronics, 20(4):807-13.

11. Bi LJ, Zhou YF, Zhang XE*, Deng JY, Zhang ZP, Xie B and Zhang CG (2003). A Muts Based Protein Chip for Detection of DNA Mutations, Analytical Chemistry, 75(16): 4113-4119.

12. Zhou YF, Zhang XE*, Liu H, Zhang CG and Cass AEG (2001), Construction of a Fusion Enzyme System by Gene Splicing as a New Molecular Recognition Element for Sequence Biosensor, Bioconjugate Chemistry, 12 (6): 924-931.