Bovine PrPC directly interacts with aB-crystalline. FEBS Letters, 2005, 579:5419-5424.

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We used a bovine brain cDNA library to perform a yeast two-hybrid assay with bovine mature PrPC as bait. The screening result showed that aB-crystalline interacted with PrPC. The interaction was further evaluated both in vivo and in vitro with different methods, such as immuno.uorescent colocalization, native polyacrylamide-gel electrophoresis, and IAsys biosensor assays. The results suggested that aB-crystalline may have the ability to refold denatured prion proteins, and provided firrst evidence that aB-crystalline is directly associated with prion protein.